Limbal Stem Cells from Aged Donors Are a Suitable Source for Clinical Application

Table 2

Primers and sequences.

Target	Gene	Accession number		Primer sequence (5′-3′)	Annealing temperature
Cytokeratin 12	CK12	NM_000223	F	TGGTCATGTTGGTCTTTGTAAC	55°C
			R	ACTTCTCTCTATGCTCTTGACA
Cytokeratin 3	CK3	NM_057088	F	GAGCGGGAACAGATCAAGAC	55°C
			R	GGTAGCTCCGCAGGTAGTTG
Cytokeratin 19	CK19	NM_002276	F	TGAGTGACATGCGAAGCCAAT	55°C
			R	ACCTCCCGGTTCAATTCTTCA
Δp63α	TP63	NM_003722.4	F	GAAACGTACAGGCAACAGCA	60°C
			R	GCTGCTGAGGGTTGATAAGC
E-cadherin	E-cad	NM_004360	F	GCCTCCTGAAAAGAGAGTGGAAG	60°C
			R	TGGCAGTGTCTCTCCAAATCCG
Wnt7a	WNT7A	NM_004625.3	F	CATAGGAGAAGGCTCACAAATGG	55°C
			R	CGGCAATGATGGCGTAGGT
PAX6	PAX6	NM_000280.4	F	ATAACCTGCCTATGCAACCC	55°C
			R	GGAACTTGAACTGGAACTGAC
RNA-18S	18S	NR_003286.2	F	TATA center	60°C
			R	TATA center

F: forward; R: reverse.