Figure 1: Characterization of differentiated cells. (a) Changes in cell morphology during the hepatic differentiation of rBM-MSCs. Scale bar: 500 μm. (b) The expression of HNF3β, AFP, HNF1α, and ALB on the last day of hepatic differentiation in each experimental group, assessed using immunocytochemistry. Scale bar: 200 μm. (c) Expression of hepatocyte-specific proteins after cell exposure to chromatin-remodeling agents. Immunocytochemistry was performed for HNF3β, AFP, CK18, ALB, HNF1α, and C/EBPα. Values represent means ± SEM. Bars with different superscripts in the certain step are different statistically (). , , and . (d) RT-PCR analyses of the temporal expression patterns of selected hepatocyte-specific genes during the hepatic differentiation of rBM-MSCs.