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Stem Cells International
Volume 2016 (2016), Article ID 3162363, 10 pages
http://dx.doi.org/10.1155/2016/3162363
Research Article

Dynamic Changes in Occupancy of Histone Variant H2A.Z during Induced Somatic Cell Reprogramming

1Department of Animal Breeding & Genetics, College of Animal Science & Technology, Nanjing Agricultural University, Nanjing 210095, China
2Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123, China
3Medical College, Hunan Normal University, Changsha 410006, China
4Jinling Hospital, Medical School of Nanjing University, Nanjing 210093, China
5State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, China

Received 8 April 2015; Accepted 25 August 2015

Academic Editor: Aster H. Juan

Copyright © 2016 Fulu Dong et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The development of induced pluripotent stem cells (iPSCs) has enabled study of the mechanisms underlying cellular reprogramming. Here, we have studied the dynamic distribution of H2A.Z during induced reprogramming with chromatin immunoprecipitation deep sequencing (ChIP-Seq). We found that H2A.Z tended to accumulate around transcription start site (TSS) and incorporate in genes with a high transcriptional activity. GO analysis with H2A.Z incorporated genes indicated that most genes are involved in chromatin assembly or disassembly and chromatin modification both in MEF and Day 7 samples, not in iPSCs. Furthermore, we detected the highest level of incorporation of H2A.Z around TSS in Day 7 samples compared to MEFs and iPSCs. GO analysis with only incorporated genes in Day 7 also displayed the function of chromatin remodeling. So, we speculate H2A.Z may be responsible for chromatin remodeling to enhance the access of transcription factors to genes important for pluripotency. This study therefore provides a deeper understanding of the mechanisms underlying induced reprogramming.