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Stem Cells International
Volume 2016 (2016), Article ID 3274054, 9 pages
http://dx.doi.org/10.1155/2016/3274054
Research Article

Evaluation of Tissue Homogenization to Support the Generation of GMP-Compliant Mesenchymal Stromal Cells from the Umbilical Cord

1St. Louis Cord Blood Bank/Cellular Therapy Laboratory, SSM Health Cardinal Glennon Children’s Hospital, St. Louis, MO 63110, USA
2Department of Pediatrics, Saint Louis University School of Medicine, St. Louis, MO 63104, USA
3SSM Health St. Mary’s Hospital, St. Louis, MO 63117, USA
4Department of Obstetrics, Gynecology and Women’s Health, Saint Louis University School of Medicine, St. Louis, MO 63104, USA

Received 23 September 2015; Revised 2 December 2015; Accepted 14 December 2015

Academic Editor: Peter Czermak

Copyright © 2016 Ryan J. Emnett et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. A. Sorrentino, M. Ferracin, G. Castelli et al., “Isolation and characterization of CD146+ multipotent mesenchymal stromal cells,” Experimental Hematology, vol. 36, no. 8, pp. 1035–1046, 2008. View at Publisher · View at Google Scholar · View at Scopus
  2. L.-L. Lu, Y.-J. Liu, S.-G. Yang et al., “Isolation and characterization of human umbilical cord mesenchymal stem cells with hematopoiesis-supportive function and other potentials,” Haematologica, vol. 91, no. 8, pp. 1017–1026, 2006. View at Google Scholar · View at Scopus
  3. K. Johnson, S. Zhu, M. S. Tremblay et al., “A stem cell-based approach to cartilage repair,” Science, vol. 336, no. 6082, pp. 717–721, 2012. View at Publisher · View at Google Scholar · View at Scopus
  4. R. Sarugaser, L. Hanoun, A. Keating, W. L. Stanford, and J. E. Davies, “Human mesenchymal stem cells self-renew and differentiate according to a deterministic hierarchy,” PLoS ONE, vol. 4, no. 8, Article ID e6498, 2009. View at Publisher · View at Google Scholar · View at Scopus
  5. K. Stenderup, J. Justesen, C. Clausen, and M. Kassem, “Aging is associated with decreased maximal life span and accelerated senescence of bone marrow stromal cells,” Bone, vol. 33, no. 6, pp. 919–926, 2003. View at Publisher · View at Google Scholar · View at Scopus
  6. S. M. Mueller and J. Glowacki, “Age-related decline in the osteogenic potential of human bone marrow cells cultured in three-dimensional collagen sponges,” Journal of Cellular Biochemistry, vol. 82, no. 4, pp. 583–590, 2001. View at Publisher · View at Google Scholar · View at Scopus
  7. D. Baksh, R. Yao, and R. S. Tuan, “Comparison of proliferative and multilineage differentiation potential of human mesenchymal stem cells derived from umbilical cord and bone marrow,” STEM CELLS, vol. 25, no. 6, pp. 1384–1392, 2007. View at Publisher · View at Google Scholar · View at Scopus
  8. R. Hass, C. Kasper, S. Böhm, and R. Jacobs, “Different populations and sources of human mesenchymal stem cells (MSC): a comparison of adult and neonatal tissue-derived MSC,” Cell Communication and Signaling, vol. 9, article 12, 2011. View at Publisher · View at Google Scholar · View at Scopus
  9. C. Capelli, E. Gotti, M. Morigi et al., “Minimally manipulated whole human umbilical cord is a rich source of clinical-grade human mesenchymal stromal cells expanded in human platelet lysate,” Cytotherapy, vol. 13, no. 7, pp. 786–801, 2011. View at Publisher · View at Google Scholar · View at Scopus
  10. I. Majore, P. Moretti, F. Stahl, R. Hass, and C. Kasper, “Growth and differentiation properties of mesenchymal stromal cell populations derived from whole human umbilical cord,” Stem Cell Reviews and Reports, vol. 7, no. 1, pp. 17–31, 2011. View at Publisher · View at Google Scholar · View at Scopus
  11. C. Mennan, K. Wright, A. Bhattacharjee, B. Balain, J. Richardson, and S. Roberts, “Isolation and characterisation of mesenchymal stem cells from different regions of the human umbilical cord,” BioMed Research International, vol. 2013, Article ID 916136, 8 pages, 2013. View at Publisher · View at Google Scholar · View at Scopus
  12. M. T. Conconi, R. D. Liddo, M. Tommasini, C. Calore, and P. P. Parnigotto, “Phenotype and differentiation potential of stromal populations obtained from various zones of human umbilical cord: an overview,” The Open Tissue Engineering and Regenerative Medicine Journal, vol. 4, no. 1, pp. 6–20, 2011. View at Publisher · View at Google Scholar · View at Scopus
  13. A. Schäffler and C. Büchler, “Concise review: adipose tissue-derived stromal cells—basic and clinical implications for novel cell-based therapies,” Stem Cells, vol. 25, no. 4, pp. 818–827, 2007. View at Publisher · View at Google Scholar · View at Scopus
  14. N. Ben Azouna, F. Jenhani, Z. Regaya et al., “Phenotypical and functional characteristics of mesenchymal stem cells from bone marrow: comparison of culture using different media supplemented with human platelet lysate or fetal bovine serum,” Stem Cell Research & Therapy, vol. 3, no. 1, article 6, 2012. View at Publisher · View at Google Scholar · View at Scopus
  15. H. Castro-Malaspina, R. E. Gay, G. Resnick et al., “Characterization of human bone marrow fibroblast colony-forming cells (CFU-F) and their progeny,” Blood, vol. 56, no. 2, pp. 289–301, 1980. View at Google Scholar · View at Scopus
  16. R. Peters, M. J. Wolf, M. van den Broek et al., “Efficient generation of multipotent mesenchymal stem cells from umbilical cord blood in stroma-free liquid culture,” PLoS ONE, vol. 5, no. 12, Article ID e15689, 2010. View at Publisher · View at Google Scholar · View at Scopus
  17. M. Dominici, K. Le Blanc, I. Mueller et al., “Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement,” Cytotherapy, vol. 8, no. 4, pp. 315–317, 2006. View at Publisher · View at Google Scholar · View at Scopus
  18. V. Sabapathy, B. Sundaram, S. Vm, P. Mankuzhy, and S. Kumar, “Human Wharton's Jelly Mesenchymal Stem Cells plasticity augments scar-free skin wound healing with hair growth,” PLoS ONE, vol. 9, no. 4, Article ID e93726, 2014. View at Publisher · View at Google Scholar · View at Scopus
  19. R. C. Schugar, S. M. Chirieleison, K. E. Wescoe et al., “High harvest yield, high expansion, and phenotype stability of CD146 mesenchymal stromal cells from whole primitive human umbilical cord tissue,” Journal of Biomedicine and Biotechnology, vol. 2009, Article ID 789526, 11 pages, 2009. View at Publisher · View at Google Scholar · View at Scopus
  20. J. Hua, J. Gong, H. Meng et al., “Comparison of different methods for the isolation of mesenchymal stem cells from umbilical cord matrix: proliferation and multilineage differentiation as compared to mesenchymal stem cells from umbilical cord blood and bone marrow,” Cell Biology International, vol. 38, no. 2, pp. 198–210, 2014. View at Publisher · View at Google Scholar · View at Scopus
  21. P. Penfornis and R. Pochampally, “Isolation and expansion of mesenchymal stem cells/multipotential stromal cells from human bone marrow,” Methods in Molecular Biology, vol. 698, pp. 11–21, 2011. View at Publisher · View at Google Scholar · View at Scopus
  22. M. C. Vemuri, L. G. Chase, and M. S. Rao, “Mesenchymal stem cell assays and applications,” Methods in Molecular Biology, vol. 698, pp. 3–8, 2011. View at Publisher · View at Google Scholar · View at Scopus
  23. M. Crisan, S. Yap, L. Casteilla et al., “A perivascular origin for mesenchymal stem cells in multiple human organs,” Cell Stem Cell, vol. 3, no. 3, pp. 301–313, 2008. View at Publisher · View at Google Scholar · View at Scopus
  24. P. Shetty, K. Cooper, and C. Viswanathan, “Comparison of proliferative and multilineage differentiation potentials of cord matrix, cord blood, and bone marrow mesenchymal stem cells,” Asian Journal of Transfusion Science, vol. 4, no. 1, pp. 14–24, 2010. View at Publisher · View at Google Scholar · View at Scopus
  25. A. Bongso and C.-Y. Fong, “The therapeutic potential, challenges and future clinical directions of stem cells from the Wharton's jelly of the human umbilical cord,” Stem Cell Reviews and Reports, vol. 9, no. 2, pp. 226–240, 2013. View at Publisher · View at Google Scholar · View at Scopus
  26. Y. Mori, J. Ohshimo, T. Shimazu et al., “Improved explant method to isolate umbilical cord-derived mesenchymal stem cells and their immunosuppressive properties,” Tissue Engineering Part C: Methods, vol. 21, no. 4, pp. 367–372, 2015. View at Google Scholar
  27. S. P. Veres, J. M. Harrison, and J. M. Lee, “Mechanically overloading collagen fibrils uncoils collagen molecules, placing them in a stable, denatured state,” Matrix Biology, vol. 33, pp. 54–59, 2014. View at Publisher · View at Google Scholar · View at Scopus
  28. E. Bańkowski, K. Sobolewski, L. Romanowicz, L. Chyczewski, and S. Jaworski, “Collagen and glycosaminoglycans of Wharton's jelly and their alterations in EPH-gestosis,” European Journal of Obstetrics Gynecology and Reproductive Biology, vol. 66, no. 2, pp. 109–117, 1996. View at Publisher · View at Google Scholar · View at Scopus
  29. N. Fekete, M. T. Rojewski, R. Lotfi, and H. Schrezenmeier, “Essential components for Ex vivo proliferation of mesenchymal stromal cells,” Tissue Engineering Part C: Methods, vol. 20, no. 2, pp. 129–139, 2014. View at Publisher · View at Google Scholar · View at Scopus
  30. N. H. Riordan, M. Madrigal, J. Reneau et al., “Scalable efficient expansion of mesenchymal stem cells in xeno free media using commercially available reagents,” Journal of Translational Medicine, vol. 13, article 232, 2015. View at Publisher · View at Google Scholar
  31. P. J. Hanley, Z. Mei, M. Da Graca Cabreira-Hansen et al., “Manufacturing mesenchymal stromal cells for phase I clinical trials,” Cytotherapy, vol. 15, no. 4, pp. 416–422, 2013. View at Publisher · View at Google Scholar · View at Scopus