Figure 4: Effect of XPC on the expression of Oct-4, Sox2, and c-Myc in DPCs at various passages. Immunofluorescent staining revealed that XPC (a1), Oct-4 (a2), Sox2 (a3), and c-Myc (a4) were strongly expressed in DPCs with XPC overexpression at P7, mainly located in nucleus and moderately expressed in cytoplasm, whereas XPC (a5), Oct-4 (a6), Sox2 (a7), and c-Myc (a8) were barely detected in DPCs at P7 without transfection. Real-time PCR indicated that the mRNA expression of XPC, Oct-4, Sox2, and c-Myc was upregulated significantly in DPCs with XPC overexpression at both P3 and P7 compared with vector groups (). There is no significant difference between nontransfected group and vector group () (b1–b3).