In Vitro Characterization of Human Mesenchymal Stem Cells Isolated from Different Tissues with a Potential to Promote Complex Bone Regeneration

Figure 2

Heterogeneity of the different MSC samples. The morphology of the cells was demonstrated with Giemsa staining (a). The colony forming ability of MSCs was investigated on 10 cm petri dishes (b). Colony forming efficiency was calculated as the number of colonies divided by the total number of seeded cells (c). The CD106 and CD146 expression in different MSC isolates determined by flow cytometry (d). The expression levels of CD90, CD73, and CD29 markers of MSC samples were determined with flow cytometry and represented as median fluorescence intensities (MFI) (e). The photos demonstrate representative samples. value less than 0.001.

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