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Stem Cells International
Volume 2016, Article ID 3604203, 9 pages
Review Article

Recent Advances and Future Direction in Lyophilisation and Desiccation of Mesenchymal Stem Cells

1Department of Biomedical Engineering, National Institute of Technology, Raipur 492010, India
2Department of Biotechnology, National Institute of Technology, Raipur 492010, India
3Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan 420000, Russia
4Preventive Medicine & Diagnosis Innovation Program (PMI), Division of Genomic Technologies, RIKEN, Yokohama 2300045, Japan
5Department of Biochemistry, Pt. JNM Medical College, Raipur 492001, India

Received 18 April 2016; Accepted 3 July 2016

Academic Editor: Andrea Ballini

Copyright © 2016 Akalabya Bissoyi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Mesenchymal Stem Cells (MSCs) are a promising mammalian cell type as they can be used for the reconstruction of human tissues and organs. MSCs are shown to form bone, cartilage, fat, and muscle-like cells under specific cultivation conditions. Current technology of MSCs cryopreservation has significant disadvantages. Alternative technologies of mammalian cells preservation through lyophilisation or desiccation (air-drying) are among the upcoming domains of investigation in the field of cryobiology. Different protectants and their combinations were studied in this context. Loading of the protectant in the live cell can be a challenging issue but recent studies have shown encouraging results. This paper deals with a review of the protectants, methods of their delivery, and physical boundary conditions adopted for the desiccation and lyophilisation of mammalian cells, including MSCs. A hybrid technique combining both methods is also proposed as a promising way of MSCs dry preservation.