Review Article
Recent Advances and Future Direction in Lyophilisation and Desiccation of Mesenchymal Stem Cells
Table 1
Advantages of trehalose over sucrose as a lyoprotectant.
| Sucrose | Trehalose |
| Vulnerable to hydrolysis under acidic conditions | Not prone to hydrolysis under acidic conditions |
| Smaller hydration radius; thus large amount of sucrose is required for lyophilisation | Hydration radius 2.5 times that of sucrose, thus requiring 2.5 times less trehalose for lyophilisation as compared to sucrose |
| Does not protect cells and proteins from oxidative damage | Protects cells and proteins from oxidative damage |
| Less interaction with water and hence does not displace water molecules bound to carbonyls at the phospholipid bilayer of the cell membrane | Interacts more strongly with water and thus able to displace water molecules bound to carbonyls at the phospholipid bilayer of cell membranes |
| Lower transition temperature | High transition temperature |
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