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Stem Cells International
Volume 2016, Article ID 3658798, 12 pages
http://dx.doi.org/10.1155/2016/3658798
Research Article

Comparison of the Biological Characteristics of Mesenchymal Stem Cells Derived from Bone Marrow and Skin

1Institute of Dermatology, Taiyuan City Centre Hospital, Shanxi Provincial Key Laboratory of Immunological Skin Diseases, No. 1 Dong San Dao Xiang, Taiyuan, Shanxi 030009, China
2Department of Dermatology, Zibo City First Hospital, Shandong 255200, China

Received 13 January 2016; Revised 18 March 2016; Accepted 6 April 2016

Academic Editor: Shinn-Zong Lin

Copyright © 2016 Ruifeng Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Mesenchymal stem cells (MSCs) exhibit high proliferation and self-renewal capabilities and are critical for tissue repair and regeneration during ontogenesis. They also play a role in immunomodulation. MSCs can be isolated from a variety of tissues and have many potential applications in the clinical setting. However, MSCs of different origins may possess different biological characteristics. In this study, we performed a comprehensive comparison of MSCs isolated from bone marrow and skin (BMMSCs and SMSCs, resp.), including analysis of the skin sampling area, separation method, culture conditions, primary and passage culture times, cell surface markers, multipotency, cytokine secretion, gene expression, and fibroblast-like features. The results showed that the MSCs from both sources had similar cell morphologies, surface markers, and differentiation capacities. However, the two cell types exhibited major differences in growth characteristics; the primary culture time of BMMSCs was significantly shorter than that of SMSCs, whereas the growth rate of BMMSCs was lower than that of SMSCs after passaging. Moreover, differences in gene expression and cytokine secretion profiles were observed. For example, secretion of proliferative cytokines was significantly higher for SMSCs than for BMMSCs. Our findings provide insights into the different biological functions of both cell types.