Three-Dimensional Gastrointestinal Organoid Culture in Combination with Nerves or Fibroblasts: A Method to Characterize the Gastrointestinal Stem Cell Niche

Figure 2

Characterization of small intestinal organoids and small intestinal myofibroblasts used to reconstruct intestinal stem cell niche ex vivo. (a) H&E staining, PAS staining, and Ki-67 staining of small intestine organoids. Scale bar, 50 μm. (b) Lgr5-GFP-positive epithelial cells in cultured conditions (marked with arrowheads). Scale bar, 100 μm. (c) Morphology of small intestine myofibroblasts, phase contrast microscopy. (d) Confirmation of the purity of small intestine organoids and small intestine myofibroblasts by Reverse-Transcription PCR. RT- (reaction without the addition of reverse transcriptase) served as the negative control.

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