Three-Dimensional Gastrointestinal Organoid Culture in Combination with Nerves or Fibroblasts: A Method to Characterize the Gastrointestinal Stem Cell Niche

<table class="table-group" id="tab1"><tr><td><table class="table"><tr><td class="thead-hr" colspan="2"><hr/></td></tr><tr class="thead"><td align="left">Culture conditions</td><td align="left">Composition</td></tr><tr><td class="thead-hr" colspan="2"><hr/></td></tr><tr><td align="left">Crypt medium (CM)</td><td align="left">Advanced DMEM/F12 + HEPES + Pen/Strep + Glutamax + growth supplements (B27, N2) + ENR (EGF, R-Spondin, and Noggin) + N-acetylcysteine</td></tr><tr><td align="center" colspan="2"><hr/></td></tr><tr><td align="left">Cardia medium (CaM)</td><td align="left">Wnt-conditioned advanced DMEM/F12 + HEPES + Pen/Strep + Glutamax + growth supplements (B27, N2) + ENR (EGF, R-Spondin, and Noggin) + N-acetylcysteine</td></tr><tr class="table-tr"><td colspan="2"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

Composition of standard media applied in the described methods.

Stem Cells International

tab1

Table 1

Table 1: Three-Dimensional Gastrointestinal Organoid Culture in Combination with Nerves or Fibroblasts: A Method to Characterize the Gastrointestinal Stem Cell Niche 

Table 1 | Three-Dimensional Gastrointestinal Organoid Culture in Combination with Nerves or Fibroblasts: A Method to Characterize the Gastrointestinal Stem Cell Niche 