Hijacking the Cellular Mail: Exosome Mediated Differentiation of Mesenchymal Stem Cells
Table 4
Four-week exosome mediated change in gene expression: 3D HMSC cultures.
Gene
HMSC regular exosomes fold change ( value)
HMSC osteogenic exosomes fold change ( value)
Growth factors
BMP2
38.26 (0.0089)
21.34 (0.0255)
BMP9
34.13 (0.0003)
66.52 (0.0001)
BMP6
19.82 (0.0010)
13.35 (0.0134)
VEGFA
33.53 (0.0105)
16.42 (0.0191)
FGF2
17.62 (0.0021)
8.85 (0.0046)
TGFB1
27.39 (0.0061)
19.77 (0.0147)
GDF10
19.42 (0.0023)
15.26 (0.0001)
Transcription factors
RUNX2
17.12 (0.0004)
10.53 (0.010216)
OSX
33.84 (0.0003)
20.86 (0.000897)
ECM proteins
OCN
10.86 (0.0298)
6.59 (0.0048)
ALPL
25.27 (0.0089)
19.28 (0.0051)
OPN
17.75 (2.54E − 05)
9.03 (0.002589)
COL1
20.80 (0.0001)
12.04 (0.0063)
Data represent fold change in gene expression when HMSCs were cultured in 3D type I collagen hydrogels in the presence of regular and osteogenic exosomes isolated from 4-week cultures. Data are presented as mean fold change in gene expression with respect to control. value specified in brackets shows statistical significance with respect to control obtained by means of Student’s -test.
