Differentiation of Bone Marrow Mesenchymal Stem Cells to Cardiomyocyte-Like Cells Is Regulated by the Combined Low Dose Treatment of Transforming Growth Factor-β1 and 5-Azacytidine

Figure 6

Protein expression levels of cTnI, α-actin, p-ErK1/2, and OCT4. (a, c) The protein expression levels of cTnI in the different doses of cocktail. versus control group; versus 3 μmol/L 5-AZA combined with 3 ng/mL TGF-β1; versus 10 μmol/L 5-AZA combined with 10 ng/mL TGF-β1. (b, d) The protein expression levels of cTnI at days 0, 7, 14, and 28 in the 5-AZA induction group and in the combination induction group. versus day 0; , versus day 7; versus day 14. (e, f) The expression levels of cTnI, α-actin, p-ErK1/2, and OCT4 proteins in different treatment groups. versus control group; , versus TGF-β1 group; versus 5-AZA group ().

(a)

(b)

(c)

(d)

(e)

(f)