PS1/γ-Secretase-Mediated Cadherin Cleavage Induces β-Catenin Nuclear Translocation and Osteogenic Differentiation of Human Bone Marrow Stromal Cells

Figure 6

PS1/γ-secretase inhibition impairs BMSCs osteogenic differentiation. BMSCs were osteoinduced in the presence of Dapt, added either 24 hours before OM (24 h Dapt + OM) or at 24 h (OM 24 h + Dapt), 48 h (OM 48 h + Dapt), or 4 days after OM (OM 4 days + Dapt). (a) Alkaline phosphatase activity measured after 7 and 14 days. Graph represents the mean ± SD of 6 independent experiments. (b–e) Von Kossa and Oil Red O double staining. (b) Control osteoinduced, (c) 24 h Dapt + OM, (d) OM 24 h + Dapt, and (e) OM 48 h + Dapt. Scale bars = 100 μm. Data are representative of 4 independent experiments. (f) Quantification of total mineralized area revealed by Von Kossa staining. Data represent mean ± SD of 3 independent experiments. . (g) Analysis of Hes-1 mRNA in BMSCs osteoinduced for 48 h and 5 days. Bars represent the mean ± SD of 3 independent experiments performed in triplicate.

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