Absence of Rybp Compromises Neural Differentiation of Embryonic Stem Cells

Figure 3

Neural markers (Nestin (a), Pax6 (b), NeuroD1 (c), Tubb3 (d), NeuN (e), Gfap (f), and Olig2 (g)) and transcription factor, Plagl1 (h), exhibit altered expression in the lack of Rybp during in vitro neural differentiation. Relative gene expression of key neural markers was analyzed with qRT-PCR. For the analysis RNA was extracted and reverse-transcribed from differentiated neural cell culture generated from and ESCs. Samples are collected at days 0, 3, 7, 10, and 14 of differentiation. The expressions of the indicated markers were normalized to Hprt expression, which was used as an internal control. Means are standard deviation. Values of were accepted as significant (; ; ). Statistical method: test type 3.

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