Er-Xian Decoction Stimulates Osteoblastic Differentiation of Bone Mesenchymal Stem Cells in Ovariectomized Mice and Its Gene Profile Analysis

Figure 2

The effect of EXD treatment on activities of osteoblasts and osteoclasts in OVX mice. (a) Representative images of H & E staining demonstrated that OVX induced a loss of the number of bone trabeculae, which was reduced by EXD (×100). (b) Morphometric data displayed a great decrease of N.Ob/T.Ar and N.Ob/B.pm in OVX mice compared to Sham mice, which was partially rescued in EXD mice with 3-mon treatment. (c, d) Representative images of Trap staining showed EXD inhibited the elevated activity of osteoclasts induced by OVX as evaluated by N.Oc/B.pm (×200). (e) Immunostaining of L₄ vertebrae with antibody against OC. Positive staining was indicated in brown (×200). (f) Quantitative data of (e). The columns represent the means ± SE per group. versus sham, ^## versus OVX.

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