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Stem Cells International
Volume 2016 (2016), Article ID 4304916, 11 pages
Research Article

Direct Reprogramming of Mouse Fibroblasts to Neural Stem Cells by Small Molecules

1School of Pharmacy and Medical Sciences, Sansom Institute, University of South Australia, Adelaide, SA 5000, Australia
2Department of Human Physiology and Centre for Neuroscience, Flinders University of South Australia, P.O. Box 2100, Adelaide, SA 5001, Australia

Received 14 April 2015; Accepted 18 August 2015

Academic Editor: Libera Berghella

Copyright © 2016 Yan-Chuang Han et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure S1. A schematic diagram depicting the protocol for the production of small molecule-induced neural stem (SMINS) cells.

Supplementary Figure S2. MEF tested by neural stem cell markers.

Supplementary Figure S3. SMINS-MEF-7 cells induced from mouse embryonic fibroblasts.

Supplementary Figure S4. The positive control native neural stem cells (NS).

Supplementary Figure S5. The staining of Alkaline Phosphatase (ALP) for NS, SMINS and Fibroblasts.

Supplementary Figure S6. Quantitative analysis of 84 neural stem cell genes by RT profiler PCR arrays.

Supplementary Figure S7. Differentiation of SMINS-MEF-7 cells in vitro.

Supplementary Figure S8. Immunocytochemistry staining of fibroblasts using nervous cell markers.

Supplementary Figure S9. The sorting of negative TTF.

Supplementary Figure S10. SMINS3 cells were check by pluripotent stem cells marker.

Supplementary Figure S11. Immunocytochemistry staining of fibroblasts and SMINS cells using a fibroblast marker.

Supplementary Figure S12. Migration of SMINS-TTF-3 cells in vivo.

Supplementary Figure S13. The majority of differentiated cells demonstrate K+ currents only.

Supplementary Table S1. Analyses results of up-regulated and down-regulated genes for SMINS-MEF-7 cells (test) vs MEF (control).

Supplementary Table S2. Analyses results of up-regulated genes for SMINS-MEF-7 cells (test) vs NS (control).

Supplementary Table S3. The primers of RT-PCR in the experiment.

  1. Supplementary Material