Aryl Hydrocarbon Receptor Deficiency in an Exon 3 Deletion Mouse Model Promotes Hematopoietic Stem Cell Proliferation and Impacts Endosteal Niche Cells

Figure 4

Lack of AHR expression in BM niche environment increases BM reconstitution ability of AHR expressing donor hematopoietic cells. Bone marrow chimera mice were prepared by lethal irradiation of WT (CD45.2⁺), WT (CD45.1⁺), and AHR-KO (CD45.2⁺) mice, as described in Section 2. Irradiated WT (CD45.1⁺) mice reconstituted with WT (CD45.2⁺) cells [WT (CD45.2⁺) WT (CD45.1⁺)], irradiated WT (CD45.2⁺) mice reconstituted with WT (CD45.1⁺) cells [WT (CD45.1⁺) WT (CD45.2⁺)], irradiated WT (CD45.1⁺) mice reconstituted with AHR-KO (CD45.2⁺) cells [AHR-KO (CD45.2⁺) WT (CD45.1⁺)], and irradiated AHR-KO (CD45.2⁺) mice reconstituted with WT (CD45.1⁺) cells [WT (CD45.1⁺) AHR-KO (CD45.2⁺)] are presented. Data shown are mean ± SD, mice/group. significantly different from WT control ().