Aryl Hydrocarbon Receptor Deficiency in an Exon 3 Deletion Mouse Model Promotes Hematopoietic Stem Cell Proliferation and Impacts Endosteal Niche Cells

Figure 5

Lack of AHR expression alters the niche population. (a) Gating of nonhematopoietic endosteal niche cells. Endosteal niche cells are separated as Lin⁻CD45⁻, endothelial cells (EC) were gated as Lin⁻CD45⁻CD31⁺ cells, and MSC (mesenchymal stem cells) and OB (osteoblast) cells were gated from the Lin⁻CD45⁻CD31⁻ population based on Sca-1 and CD51 markers. The MSC are Lin⁻CD45⁻CD31⁻Sca-1⁺CD51⁺ and OB cells are Lin⁻CD45⁻CD31⁻Sca-1⁻CD51⁺. (b) represents the percentage of the endosteal niche cells from total endosteal BM stromal cells. Data are mean ± SD, mice/group. significantly different from WT control ().

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