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Stem Cells International
Volume 2016, Article ID 4536187, 12 pages
Research Article

Aryl Hydrocarbon Receptor Deficiency in an Exon 3 Deletion Mouse Model Promotes Hematopoietic Stem Cell Proliferation and Impacts Endosteal Niche Cells

Department of Environmental Medicine, University of Rochester Medical Center, Rochester, NY 14642, USA

Received 27 January 2016; Revised 29 March 2016; Accepted 7 April 2016

Academic Editor: Armand Keating

Copyright © 2016 Zeenath Unnisa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

AHR also regulates HSC differentiation in transplantation recipient animals. We performed serial transplantation assay to assess the role of AHR in differentiation and homing of HSCs. We analyzed the different lineages in the spleen and bone marrow of the transplantation recipient animals after 16 weeks post transplantation. We found a significant increase in the CD3 and decrease in B220 lineages of the AHR KO spleens of tertiary recipients. The bone marrow showed decrease in B220 and increase in Gr1 and Mac1 lineages. These changes in BM reflect myeloid-biased differentiation which is classical sign of aging. The myeloid-biased HSCs have extensive self-renewal capacity but diminished ability to differentiate to lymphoid cells (Supplementary Figure 1). Histological examination of spleen showed increased cellularity and lymphoid follicle number in AHR KO mice. Increased thickness of cortical area in thymus and portal fibrosis in livers of AHR KO mice was also observed (Supplementary Figure 2).

  1. Supplementary Material