Research Article

Tissue-Related Hypoxia Attenuates Proinflammatory Effects of Allogeneic PBMCs on Adipose-Derived Stromal Cells In Vitro

Figure 2

ASC immunophenotype after monoculture and transwell coculture with PHA-stimulated PBMCs under 20% and 5% O2. (a) Representative histograms of ASC surface marker expression. The white filled histograms indicate the positively stained cells while the grey filled histograms indicate the isotype-matched antibody controls. (b) CD90 mean fluorescence intensity (MFI) on cocultured ASCs versus ASCs in monoculture. (c) The proportion of CD54-positive ASCs in monoculture and transwell coculture with PBMCs. (d) CD54 MFI on cocultured ASCs versus ASCs in monoculture. Significant difference between monoculture and coculture at 20% O2 (). Significant difference between monoculture and coculture at 5% O2 ().
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