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Stem Cells International
Volume 2016, Article ID 4834616, 12 pages
Research Article

Manufacturing of Human Umbilical Cord Mesenchymal Stromal Cells on Microcarriers in a Dynamic System for Clinical Use

1Department of Anatomy and Physiology, Kansas State University, 228 Coles Hall, Manhattan, KS 66506, USA
2Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, Wiesenstraße 14, 35390 Gießen, Germany
3Department of Chemical Engineering, Kansas State University, 1005 Durland Hall, Manhattan, KS 66506, USA
4Faculty of Biology and Chemistry, Justus Liebig University, Ludwigstraße 23, 35390 Gießen, Germany
5Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Project Group Bioresources, Winchester Straße 2, 35392 Gießen, Germany

Received 7 October 2015; Revised 28 December 2015; Accepted 29 December 2015

Academic Editor: Renke Li

Copyright © 2016 Florian Petry et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplemental Figure 1: Surface marker analysis. Flow cytometry data represented in histograms, blue = test, red = isotype control. Negative (isotype control) gate was set to include 99% of the isotype; the positive gate percentages are shown for each sample. hUC MSCs were positive for A) CD90 stain B) CD105 stain C) CD73 stain, and negatively stained for the Negative cocktail (e.g., a mixture of CD34, CD45, CD11b, CD19, and HLA-DR). Note that CD44 was as a positive control (in black) for the negative cocktail and was used to set the negative control gate. E) CD 44 marker included as positive cocktail.

Supplemental Figure 2: Colony forming efficiency (CFE) at day 6 of incubation after plating at 5 or 10 cells∙cm-2. Data averaged from technical quadruplicates. CFE is defined as the number of plated cells divided by the number of colonies.

Supplemental Figure 3: Normal female karyotype was observed for MSCs passage 5 from HUC#255 following cultivation in spinner flask.

Supplemental Table 1: Metabolite Yields. Lactate and ammonium yields for hUC MSCs grown in static 12-well culture plates or dynamic spinner culture on microcarriers.

  1. Supplementary Material