Wharton’s Jelly-Derived Mesenchymal Stromal Cells and Fibroblast-Derived Extracellular Matrix Synergistically Activate Apoptosis in a p21-Dependent Mechanism in WHCO1 and MDA MB 231 Cancer Cells In Vitro

Figure 5

MSCs and matrix-mediated downregulation of WHCO1 and MDA MB 231 cellular gene expression occurs via a p21 pathway. (a) Western blot analysis of lysates from WHCO1 and MDA MB 231 cells cocultured with MSCs and transfected with p53 siRNA for 48 hrs showing expression of p53, p21, PCNA, Bcl-2, MMP-2, and Bcl-xL. (b) Western blot analysis of lysates from WHCO1 and MDA MB 231 cells cocultured with MSCs and transfected with p21 siRNA for 48 hrs showing expression of the same genes as in (a). (c) Western blot analysis of lysates from WHCO1 and MDA MB 231 cells cocultured with MSCs and transfected with p53 siRNA for 48 hrs showing expression of Ki67. (d) Western blot analysis of lysates from WHCO1 and MDA MB 231 cells cocultured with MSCs and transfected with p21 siRNA for 48 hrs showing expression of Ki67. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a loading control.

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