Human iPSC for Therapeutic Approaches to the Nervous System: Present and Future Applications

Figure 1

Diagrammatic representation of methods used to obtain human iPSC. Different somatic cells can be used for reprogramming (left column). Reprogramming techniques (center column) first used viral based genomic integration (a) and then used footprint-free techniques (b). Footprint-free iPSC induction can be obtained by Sendai virus (b(i)); episome (b(ii)); mRNA (b(iii)); siRNA (b(iv)). Finally, culturing conditions (right column) at first requiring feeder cells evolved to xeno-free conditions to allow safer clinical translation.