Neurosphere Based Differentiation of Human iPSC Improves Astrocyte Differentiation
Increased astrocyte differentiation mediated by neurospheres. (a) ICC of 21-day differentiated astrocytes (AQP4) derived from 2D and 3D NPCs. (b) Quantification of AQP4+ positive astrocytes derived from 2D and 3D NPCs. Results were reported as Mean ± SEM of 15 fields from 3 independent cultures (), using Student’s -test. (c) Analysis of gene expression by qPCR of AQP4 and TBR2. The expression values were normalized to GAPDH gene expression. Subsequently, the expression values were calculated as relative amount of mRNA versus expression values of N S5 (derived from 2D NPCs) 21-day differentiated cells, which was set to 1. Data was reported as Mean ± SEM of 3 independent cultures, tested for significant difference () using Student’s -test.
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