Research Article
Identification of New Rat Bone Marrow-Derived Population of Very Small Stem Cell with Oct-4A and Nanog Expression by Flow Cytometric Platforms
Figure 2
Expression of mRNA for Oct-4, Nanog, and Rexo-1 by real-time RT-PCR in sorted fractions of BM cells. Indicated populations of nonhematopoietic (CD45−/Lin−/CD106+, CD45−/Lin−/CD106+/CD54+, and CD45−/Lin−/SSEA-1+) and hematopoietic (CD45+/Lin−/CD106+, CD45+/Lin−/CD106+/CD54+) cells were sorted with MoFlo XDP. The graphs show the fold difference in concentration of mRNA for Oct-4, Nanog, and Rexo-1 in sorted fractions when compared to unfractionated TNCs (shown as 1). Results are presented as mean ± SD. Statistically significant differences () are shown when compared with TNCs. Analysis was performed three times with samples prepared from three independent sorts.