Identification of New Rat Bone Marrow-Derived Population of Very Small Stem Cell with Oct-4A and Nanog Expression by Flow Cytometric Platforms

Table 2

Quantitative real time RT-PCR analysis of mRNA expression for genes related to pluripotency in purified populations of rat BM-derived populations.

Cells fractions	Pluripotent stem cells markers
	Oct-4	Nanog	Rexo1
	Mean ± SD	Mean ± SD	Mean ± SD
Unfractionated TNCs (control)	1.00	1.00	1.00
CD45^−/Lin^−/SSEA-1^+	4.58 ± 0.91	15.08 ± 6.67	7.90 ± 3.11
CD45^−/Lin^−/CD106^+	34.30 ± 17.35	103.89 ± 27.56	9.34 ± 3.51
CD45^+/Lin^−/CD106^+	2.16 ± 0.91	8.63 ± 2.88	4.90 ± 2.00
CD45^−/Lin^−/CD106^+/CD54^+	3.11 ± 0.31	13.07 ± 2.72	8.15 ± 2.66
CD45^+/Lin^−/CD106^+/CD54^+	8.54 ± 3.85	6.69 ± 0.99	4.87 ± 1.73

Results are presented as values of ΔΔCt (average data based on 3 independent experiments; mean ± SD) when compared with whole unfractionated BM tissue (control; computed as 1.00).