Research Article

Identification of New Rat Bone Marrow-Derived Population of Very Small Stem Cell with Oct-4A and Nanog Expression by Flow Cytometric Platforms

Table 3

Comparison of major features and phenotype of human and murine VSELs with rat BM-derived VSELs.

FeatureHuman VSELs
[3, 4, 12, 46]
Murine VSEL
[1, 5, 12, 15, 2325]
Rat VSELs

Morphology of “very small SCs”
Cells smaller than red blood cells in researched typical species contain large nuclei surrounded by a narrow rim of cytoplasm
+++

Size (by ISS)6.75 ± 1.04 µm3.63 ± 0.27 µm5.32 ± 0.39 µm

Frequency (% of TNCs)0.01%0.02%0.03%

Phenotype currently used for purification (FACSCD45/Lin/CD133/1+
CD45/Lin/CD34+
CD45/Lin/Sca-1+CD45/Lin/CD106+

Other surface antigen expressionCD133/1+ (AC133+), CD34+, SSEA-4+, AP+, c-Met+, LIF-R+, CXCR4+, CD45, LinSca-1+, SSEA-1+, AP+, c-Met+, LIF-R+, CXCR4+, CD45, Lin, HLA-DR, MHC I CD90, CD29, CD105CD106+, CD54+/−, SSEA-1+, CD45, Lin

Expression of pluripotent SC transcription factors: Oct-4A and Nanog+++

() Lin – abbreviation indicating major markers characterizing main fractions of hematopoietic cell lineages present in BM tissue such as erythrocytes, monocytes, granulocytes, and subfractions of lymphocytes.