Mesenchymal Stem/Stromal Cells from Discarded Neonatal Sternal Tissue: In Vitro Characterization and Angiogenic Properties

Figure 3

Trilineage differentiation of neonatal sbMSCs. Sternal MSCs ( lines) were incubated with differentiation media for 14–21 days. (a) Osteogenic differentiation was demonstrated by anti-osteocalcin staining. (b) Chondrogenic differentiation by anti-aggrecan staining. (c and d) Adipogenic differentiation was demonstrated by Oil Red O and anti-FABP. (e–h) Neonatal sbMSCs cultured in standard growth media did not demonstrate any spontaneous differentiation. (i) Varying efficiency of adipogenic differentiation of sbMSCs by Oil Red O staining as quantified by image analysis. Data were analyzed by one-way ANOVA with post hoc Tukey test and significant differences () are indicated with brackets.

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