Research Article

Neurotrophic Effect of Adipose Tissue-Derived Stem Cells on Erectile Function Recovery by Pigment Epithelium-Derived Factor Secretion in a Rat Model of Cavernous Nerve Injury

Figure 5

EdU labeling of ADSCs and tracking of EdU-labeled ADSCs in the penile tissues after cell transplantation. (a) Representative image of ADSCs labeled with EdU at 10 μM and stained with Alexa Fluor 594 (red fluorescence) and Hoechst 33342 (blue fluorescence). Original magnification is 200x. (b)–(d) Penis tissues were harvested at 7 d, 14 d, and 28 d after ADSCs transplantation and stained with Alexa Fluor 594 (red fluorescence) and DAPI (blue fluorescence). The merged image showed that the EdU and DAPI double positive cells displayed a time-dependent decreasing pattern in the penis tissue. Original magnification is 200x. EdU = 5-ethynyl-2-deoxyuridine; DAPI = 4′,6-diamidino-2-phenylindole.