Phenotypic and Functional Characterization of Mesenchymal Stem/Multipotent Stromal Cells from Decidua Basalis of Human Term Placenta

Figure 1

Photomicrographs showing representative examples of DBMSCs at passage three (a) and a colony forming unit of DBMSCs isolated from passage three (b). The differentiation of DBMSCs isolated from passage three into adipocytes shown by HCS LipidTOX Green neutral lipid staining of adipocytes after 21 days (c); osteocytes were shown by Alizarin Red S staining of osteocytes after 21 days (d) and chondrocytes shown by Alcian Blue staining of cross-section of a chondrogenic pellet after 21 days (e). Negative control of DMSC differentiation into adipocytes (f), osteocytes (g), and chondrocytes (h). Scale bars represent 100 μm.

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