Antimicrobial Properties of Mesenchymal Stem Cells: Therapeutic Potential for Cystic Fibrosis Infection, and Treatment
MSCs products decrease Pseudomonas aeruginosa growth. Bone marrow derived hMSCs supernatants were cultured with different dosages of Pseudomonas aeruginosa with and without the addition of geneticin (100 μg/mL). Aliquots of the bacteria were streaked onto TSA plates for CFUs (a) or evaluated for ATP production (b). hMSC supernatants ( different donors) significantly decreased both Pseudomonas aeruginosa growth kinetics () and CFUs (). Geneticin was used as a positive control which also significantly decreased both CFUs () and growth rate () which was enhanced by the addition of hMSCs ( versus antibiotic alone for both CFUs and growth kinetics). PA = Pseudomonas aeruginosa growth without treatment. Geneticin = treatment with the antibiotic geneticin, +hMSCs = treatment with hMSC derived supernatant, and Both = treatment with both hMSC supernatants and geneticin.
|(a) Pseudomonas aeruginosa CFUs|
|(b) Pseudomonas aeruginosa growth rate|