Research Article

Antimicrobial Properties of Mesenchymal Stem Cells: Therapeutic Potential for Cystic Fibrosis Infection, and Treatment

Figure 2

MSCs products decrease Pseudomonas aeruginosa growth. Bone marrow derived hMSCs supernatants were cultured with different dosages of Pseudomonas aeruginosa with and without the addition of geneticin (100 μg/mL). Aliquots of the bacteria were streaked onto TSA plates for CFUs (a) or evaluated for ATP production (b). hMSC supernatants ( different donors) significantly decreased both Pseudomonas aeruginosa growth kinetics () and CFUs (). Geneticin was used as a positive control which also significantly decreased both CFUs () and growth rate () which was enhanced by the addition of hMSCs ( versus antibiotic alone for both CFUs and growth kinetics). PA = Pseudomonas aeruginosa growth without treatment. Geneticin = treatment with the antibiotic geneticin, +hMSCs = treatment with hMSC derived supernatant, and Both = treatment with both hMSC supernatants and geneticin.
(a) Pseudomonas aeruginosa CFUs
(b) Pseudomonas aeruginosa growth rate