tPA-MMP-9 Axis Plays a Pivotal Role in Mobilization of Endothelial Progenitor Cells from Bone Marrow to Circulation and Ischemic Region for Angiogenesis

Figure 4

Serial changes of Laser Doppler scanning of hind limb blood flow prior to and after critical limb ischemia (CLI) induction. (a–d) The normal blood flow in both hind limbs in each group prior to the CLI procedure. (e) Ratio of left (ischemia) to right (normal) blood flow, . (f–u) The blood flow in left and right hind limbs under the treatment conditions of CLI and CLI + tPA in 4 groups (i.e., B B, M B, M M, and B M) at day 2 or day 14 after the procedure. (v) Quantitation and calculation of ratio of ischemia to normal blood flow (INBF) among the four groups at day 2. (w) Quantitation and calculation of ratio of INBF among the four groups at day 14. Statistical analysis for individual group at different treatment conditions. (1) In B B, 1A versus 1B, indicates . (2) In M B, 2A versus 2B, . (3) In M M, 3A versus 3B, . (4) In B M, 4A versus 4B, indicates . (2) Statistical analysis for group comparison with respective treatment condition. (1) For CLI subgroup (white bar chart), groups with different red symbols (, ), . (2) For CLI + tPA subgroup (black bar chart), groups with different blue symbols (, ), . SC = sham control; tPA = tissue plasminogen activator. for each group.