tPA-MMP-9 Axis Plays a Pivotal Role in Mobilization of Endothelial Progenitor Cells from Bone Marrow to Circulation and Ischemic Region for Angiogenesis

Figure 6

Immunofluorescent (IF) microscopic findings (400x) of stromal cell-derived factor- (SDF-) 1α+ cells in ischemic quadriceps at day 14 after critical limb ischemia (CLI) induction. (a–p) IF microscopic findings of SDF-1α+ cells in ischemic area in four treatment conditions of four groups. (1) Statistical analysis for subgroups of each group. (1) In B B group, subgroups with different black symbols (, , and ), . (2) In M B group, subgroups with different black symbols (, ), . (3) In M M group, . (4) In B M group, subgroups with different black symbols (, , and ), . (2) Statistical analysis for group comparison with respective treatment condition. (1) For SC subgroup (red bar chart), groups with different red symbols (, ), . (2) For CLI subgroup (blue bar chart), groups with different blue symbols (, , and ), . (3) For SC + tPA subgroup (green bar chart), groups with different green symbols (, ), . (4) For CLI + tPA subgroup (orange bar chart), groups with different orange symbols (, ), . All statistical analyses were performed by one-way ANOVA followed by Bonferroni multiple comparison post hoc test. SC = sham control; tPA = tissue plasminogen activator. for each group.