RLSCs grown on soft substrate display epithelial/hepatic gene expression and hepatic function. (a) RT-qPCR analysis for the indicated genes of RLSCs grown on 0.4 kPa and 80 kPa at 24 hours (left panel) and 48 hours (right panel). Data are expressed as fold change in gene expression in cells grown on hydrogels versus CTRL (arbitrary value = 1). The graphics are representative of three independent experiments. Note the logarithmic scale. (b) Urea production in RLSCs. Urea levels in supernatant of cells grown on plastic (CTRL) and on 0.4 kPa hydrogel were analysed at 24 and 48 hours. The mean ± SD of two independent experiments is shown.