Stem Cells International / 2016 / Article / Fig 5

Research Article

Modulating the Substrate Stiffness to Manipulate Differentiation of Resident Liver Stem Cells and to Improve the Differentiation State of Hepatocytes

Figure 5

Soft substrate improves the differentiation state of hepatocyte cell lines. (a) Phase-contrast micrographs of MMH/E14 hepatocyte cell lines grown on plastic (CTRL) and on 0.4 kPa hydrogel for 24 and 48 hours. Images are representative of three independent experiments. Scale bar: 100 μm. (b) RT-qPCR analysis for the indicated genes. Data are expressed as fold change in gene expression in cells grown on 0.4 kPa versus CTRL (arbitrary value = 1). The graphic represents the mean of three independent experiments ± SD. Asterisks indicate values in Student’s -test (, , and ). Note the logarithmic scale. (c) Urea production in MMH/E14 hepatocytes. Urea levels in supernatant of cells grown on plastic (CTRL) and on 0.4 kPa hydrogel at 24 and 48 hours were analysed. The mean ± SD of two independent experiments is shown. (d) Phase-contrast micrographs of WT/3A hepatocyte cell lines grown on plastic (CTRL) and on 0.4 kPa hydrogel for 48 hours. Images are representative of three independent experiments. Scale bar: 100 μm. (e) RT-qPCR analysis for the indicated genes. Data are expressed as fold change in gene expression in cells grown on 0.4 kPa versus CTRL (arbitrary value = 1). The graphic represents the mean of three independent experiments. , , and . Note the logarithmic scale. (f) Urea production in WT/3A hepatocytes. Urea levels in supernatant of cells grown on plastic (CTRL) and on 0.4 kPa hydrogel at 48 hours were analysed. The mean ± SD of two independent experiments is shown.
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