Bone Anabolic Effects of Soluble Si: In Vitro Studies with Human Mesenchymal Stem Cells and CD14+ Osteoclast Precursors

Figure 2

Effects of Si on the differentiation and function of hMSC. (a) ALP activity, determined by pNPP hydrolysis assay. (b) qPCR analysis of cell cultures maintained in the absence (control) or presence of Si (25 μM); (c) presence of phosphate and calcium deposits in cell layers, by von Kossa and alizarin red staining, respectively. Significantly different than the control. Black bars represent 300 μm.

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