Research Article

Allogeneic Mesenchymal Stem Cell Treatment Induces Specific Alloantibodies in Horses

Figure 2

Representative flow cytometric contour plots of serum antibody binding to equine MSCs (eye study, (a)–(c); tendon study, (d)–(f)). (a) Percent positive antibody binding to MSCs prior to any in vivo MSC administration (background binding). (b) Percent positive antibody binding to irrelevant (nondonor) MSCs after in vivo MSC administration (nonspecific binding). (c) Percent positive antibody binding to donor MSCs 2 weeks after final in vivo MSC administration. (d) Percent positive antibody binding to MSCs prior to any in vivo MSC administration (background binding). (e) Percent positive antibody binding to irrelevant (nondonor) MSCs after in vivo MSC administration (nonspecific binding). (f) Percent positive antibody binding to donor MSCs 2 weeks after final in vivo MSC administration.