Ciprofloxacin Improves the Stemness of Human Dermal Papilla Cells

Figure 5

Effects of CIP on stem cell-like phenotypes in primary human DPCs. (a) Cells were treated with CIP (0–10 μg/mL) for 24 h. Cytotoxicity was determined by MTT assay. The data represent the means of four independent triplicate samples ± SD. (b) After indicated treatment, mode of cell death was examined by Hoechst 33342/PI costaining assay. Scale bar is 100 μm. ((c)–(e)) Cells were treated with CIP (0–10 μg/mL) for 72 h. After indicated treatment, the levels of stem cell markers (procollagen type I, CD133, integrin β1, and ALDH1A1), Wnt/β-catenin signaling (Akt, p-Akt, GSK3β, p-GSK3β, and β-catenin), and EMT transcription factors (ZEB1, Slug, and Snail) were determined by western blot analysis, respectively. β-actin was served as the loading control. The immunoblot signals were quantified by densitometry and mean data from independent experiments were normalized to the results. The data represent the means of four independent samples ± SD. and versus untreated control.

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