Review Article

Features of Microsystems for Cultivation and Characterization of Stem Cells with the Aim of Regenerative Therapy

Table 1

Summarization of the cell operational principles, target cells, characteristics, performance, and regenerative therapy.

MethodsOperational principlesTarget cellCharacteristicsPerformanceRegenerative therapyReference

MicrowellBiochemical reactionHematopoietic stem cell (HSCs)HydrogelProteins characteristic[24]
Human embryonic stem cell (hESCs)HydrogelDifferentiationCardiac[27]
Human embryonic stem cell (hESCs)MatrigelUndifferentiation[28]
Embryonic stem cells (ESCs)Parylene-C stencilCoculture[29]
Embryonic stem cells (ESCs)Pressing/rollingUniform-sized spheroidCardiac[32]

MicropatternSurface topologyHematopoietic stem cell (HSCs)MicropillarCell adhesion/expansion[34]
Human mesenchymal stem cell (hMSCs)MicropillarDifferentiationOsteogenic[33]
Mesenchymal stem cell (MSCs)Micro/nanopillarDifferentiationOsteogenic[35]
Human adipose-derived stem cell (hASCs)NanopillarDifferentiationOsteogenic[36]
Human mesenchymal stem cell (hMSCs)PVA patternDifferentiationVascular[37]
Mesenchymal stem cells (MSCs)PCL filmDifferentiationVascular[38]

Microfluidic channelDiffusion gradientHuman mesenchymal stem cells (hMSCs)Osmotic pump/Y-channelFBS effects on hESCs[56]
hESCs-derived neural progenitor cellsOsmotic pump/T-channelDifferentiationNeurons[57]
Human neural stem cell (hNSCs)HydrogelDifferentiationNeurons[58]
Embryonic stem cells (ESCs)Peristaltic pump/Y-channelDifferentiationNeurons[59]
Shear stressEmbryonic stem cells (ESCs)Syringe pump/valvesCells morphology[67]
Human embryonic stem cell (hESCs)Syringe pump/hydrogelDifferentiationVascular[63]
Human embryonic stem cell (hESCs)Syringe pumpCoculture[65]