Review Article
Features of Microsystems for Cultivation and Characterization of Stem Cells with the Aim of Regenerative Therapy
Table 1
Summarization of the cell operational principles, target cells, characteristics, performance, and regenerative therapy.
| Methods | Operational principles | Target cell | Characteristics | Performance | Regenerative therapy | Reference |
| Microwell | Biochemical reaction | Hematopoietic stem cell (HSCs) | Hydrogel | Proteins characteristic | — | [24] | Human embryonic stem cell (hESCs) | Hydrogel | Differentiation | Cardiac | [27] | Human embryonic stem cell (hESCs) | Matrigel | Undifferentiation | — | [28] | Embryonic stem cells (ESCs) | Parylene-C stencil | Coculture | — | [29] | Embryonic stem cells (ESCs) | Pressing/rolling | Uniform-sized spheroid | Cardiac | [32] |
| Micropattern | Surface topology | Hematopoietic stem cell (HSCs) | Micropillar | Cell adhesion/expansion | — | [34] | Human mesenchymal stem cell (hMSCs) | Micropillar | Differentiation | Osteogenic | [33] | Mesenchymal stem cell (MSCs) | Micro/nanopillar | Differentiation | Osteogenic | [35] | Human adipose-derived stem cell (hASCs) | Nanopillar | Differentiation | Osteogenic | [36] | Human mesenchymal stem cell (hMSCs) | PVA pattern | Differentiation | Vascular | [37] | Mesenchymal stem cells (MSCs) | PCL film | Differentiation | Vascular | [38] |
| Microfluidic channel | Diffusion gradient | Human mesenchymal stem cells (hMSCs) | Osmotic pump/Y-channel | FBS effects on hESCs | — | [56] | hESCs-derived neural progenitor cells | Osmotic pump/T-channel | Differentiation | Neurons | [57] | Human neural stem cell (hNSCs) | Hydrogel | Differentiation | Neurons | [58] | Embryonic stem cells (ESCs) | Peristaltic pump/Y-channel | Differentiation | Neurons | [59] | | | | | | | Shear stress | Embryonic stem cells (ESCs) | Syringe pump/valves | Cells morphology | — | [67] | Human embryonic stem cell (hESCs) | Syringe pump/hydrogel | Differentiation | Vascular | [63] | Human embryonic stem cell (hESCs) | Syringe pump | Coculture | — | [65] |
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