Subculture of Germ Cell-Derived Colonies with GATA4-Positive Feeder Cells from Neonatal Pig Testes

Table 1

Number of cells collected from colonies in subculture with fresh feeder cells.

Passage	Number of collected cells from colonies	Number of used cells for subculture
0	1.95 ± 0.03 × 10^6/12 wells	2 × 10^4/well
1	6.6 ± 0.9 × 10^5/12 wells	2 × 10^4/well
2	5.9 ± 0.6 × 10^5/12 wells	2 × 10^4/well
3	8.7 ± 0.9 × 10^5/12 wells	2 × 10^4/well
4	1.14 ± 0.12 × 10^6/12 wells	2 × 10^4/well
5	1.12 ± 0.11 × 10^6/12 wells	2 × 10^4/well
6	1.01 ± 0.26 × 10^6/12 wells	2 × 10^4/well
7	1.06 ± 0.12 × 10^6/12 wells	2 × 10^4/well
8	1.61 ± 0.36 × 10^6/12 wells	2 × 10^4/well
9	1.25 ± 0.2 × 10^6/12 wells	2 × 10^4/well
10	1.31 ± 0.58 × 10^6/12 wells	2 × 10^4/well