Stem Cells International

Research Article

Synovial Sarcoma Microvesicles Harbor the SYT-SSX Fusion Gene Transcript: Comparison of Different Methods of Detection and Implications in Biomarker Research

Table 1

Comparison of sensitivity of nested PCR, qPCR, nested PCR, and ddPCR in the detection of the SYT-SSX2 fusion gene in synovial sarcoma cells. D: detected, ND: not detected.
Amount of total RNA in ng (synovial sarcoma cells)Nested qPCRqPCRNested PCRddPCR
25,000DDDD
12,500DDDD
6,250DDDND
3,125DDDND
1,562DDNDND
0,781DDNDND
0,391DNDNDND
0,195DNDNDND
0,098NDNDNDND
0,049NDNDNDND
0,024NDNDNDND
0,012NDNDNDND
0,006NDNDNDND
0,003NDNDNDND