Scaffold-Free Fabrication of Osteoinductive Cellular Constructs Using Mouse Gingiva-Derived Induced Pluripotent Stem Cells
Expression of osteogenic marker genes in the GF-iPSC constructs under osteogenic induction. Expression of Runx2 (a), osterix (b), collagen 1a1 (c), and osteocalcin (d) on day 10, 20, and 30 was determined by quantitative real-time RT-PCR. Gene expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal control. The data represent the mean values ± SD (). Significant differences (: ANOVA with Dunnett’s correction for multiple comparisons) were evaluated with respect to day 0 (before osteogenic induction) values.