Scaffold-Free Fabrication of Osteoinductive Cellular Constructs Using Mouse Gingiva-Derived Induced Pluripotent Stem Cells

Table 1

Primers used for SYBR Green quantitative RT-PCR.

Description (gene name)	Primers (Fw, forward; Rv, reverse)	Product size (bp)	Accession number (reference)
Runx2 (Runx2)	Fw: 5′-CGGGCTACCTGCCATCAC-3′	78	NM_001146038.2 (Speer et al. [19])
	Rv: 5′-GGCCAGAGGCAGAAGTCAGA-3′
osterix (Sp7)	Fw: 5′-CTCGTCTGACTGCCTGCCTAG-3′	84	NM_130458.3 (Fowlkes et al. [20])
	Rv: 5′-GCGTGGATGCCTGCCTTGTA-3′
collagen 1a1 (Col1a1)	Fw: 5′-TGTCCCAACCCCCAAAGAC-3′	92	NM_007742.3 (Kaback et al. [21])
	Rv: 5′-CCCTCGACTCCTACATCTTCTGA-3′
osteocalcin (Bglap)	Fw: 5′-CCGGGAGCAGTGTGAGCTTA-3′	68	NM_007541.3 (Jadlowiec et al. [22])
	Rv: 5′-AGGCGGTCTTCAAGCCATACT-3′
GAPDH (Gapdh)	Fw: 5′-TGCACCACCAACTGCTTAG-3′	177	NM_001289726.1 (Gautier et al. [23])
	Rv: 5′-GGATGCAGGGATGATGTTC-3′