Research Article
Validation of Housekeeping Genes to Study Human Gingival Stem Cells and Their In Vitro Osteogenic Differentiation Using Real-Time RT-qPCR
Table 2
The 4 algorithms used in the study.
| Analysis method | PCR values | Stability values | Type of analysis |
| The ΔCt comparative method |
Ct | StdDev mean | Intergene |
| GeNorm | | , NF | Intergene One HKG all other HKGs Stable HKGs: lowest variability: low |
| BestKeeper | Ct | SD, | Intra- and intergene Pairwise correlation analysis BestKeeper index Stable HKGs: low SD and high |
| NormFinder | | | Intra- and intergene; stable HKGs: least intra- and intergroup variations |
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