p53-Dependent Senescence in Mesenchymal Stem Cells under Chronic Normoxia Is Potentiated by Low-Dose γ-Irradiation

Figure 2

Effects of oxygen levels on sensitivity of p53 wild-type mMSCs for radiation-induced senescence. (a) Green SA β-gal positive mMSCs in sham-irradiated (0 Gy) hypoxic (upper left) and in normoxic cultures (upper right) and 7 days after exposure to 4 Gy γ-irradiation (hypoxia, lower left, and normoxia, lower right). (b) Percentage of cells undergoing senescence in hypoxic and normoxic conditions after increasing doses of γ-irradiation depicted as mean values ± standard error of the mean (4 biological replicates), radiation dose on logarithmic scale.

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