Research Article

Osteogenic Differentiation of Human Amniotic Fluid Mesenchymal Stem Cells Is Determined by Epigenetic Changes

Figure 2

Gene expression analysis during osteogenic differentiation of AF-MSCs. (a) Relative alkaline phosphatase (ALP) and osteopontin, markers of osteogenic progenitors, gene expression. (b) Relative expression of genes, regulating pluripotency in stem cells: Sox2 and Rex1. Gene expression was determined by RT-qPCR and data, normalized to GAPDH, are presented as -fold change over control. Results are shown as mean ± SD (); , , and indicate significant differences from control.