Labeling Adipose-Derived Stem Cells with Hoechst 33342: Usability and Effects on Differentiation Potential and DNA Damage

Figure 3

Labeling stability (percentage of labeled cells, a) of ASCs labeled with 0.5 μg/mL or 5 μg/mL H33342 over a period of 8 weeks. Coculture of labeled and unlabeled ASCs on cover slips (b): representative images with an exposure time of 200 ms after 8 days of coculture. In the left column, ASCs labeled with 0.5 μg/mL (upper picture) and 5 μg/mL H33342 (lower picture) are shown. The right column shows formerly unlabeled ASCs that were cocultured on the same cover slip. Scale bar represents 200 μm.

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(b)