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Research Article
Enhanced Proliferation of Porcine Bone Marrow Mesenchymal Stem Cells Induced by Extracellular Calcium is Associated with the Activation of the Calcium-Sensing Receptor and ERK Signaling Pathway
Figure 1
Extracellular calcium () promoted pBMSCs proliferation and CaSR expression. (a) Effect of various (1, 2, 4, and 6 mM) on the proliferation of pBMSCs (). (b) Effect of 4 mM on the mRNA expression of CaSR in pBMSCs after 5-day culture. (c) Western blot analysis of CaSR in pBMSCs after 5-day culture. β-actin was used as loading control. (d) Mean ± SEM of immunoblotting bands of CaSR; the intensities of the bands were expressed as the arbitrary units (). , , and versus 1 mM group (control).