Stem Cells International / 2016 / Article / Fig 3

Research Article

Enhanced Proliferation of Porcine Bone Marrow Mesenchymal Stem Cells Induced by Extracellular Calcium is Associated with the Activation of the Calcium-Sensing Receptor and ERK Signaling Pathway

Figure 3

CaSR inhibition blocked the increase of in response to during the proliferation of pBMSCs. (a) of live cells were analyzed by flow cytometry. The percentage of gated positive cells represented the cells stained with Fluo 3-AM. Representation of three independent experiments with similar results. (b) The relative FITC fluorescence intensity of gated positive cells. (c) Western blot analysis of CaSR in pBMSCs after 5-day culture in the presence of 4 mM and/or 0.1 μM NPS2143. β-actin was used as loading control. (d) Mean ± SEM of immunoblotting bands of CaSR; the intensities of the bands were expressed as the arbitrary units (). versus 1 mM group (control); and versus 4 mM group.

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